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Scientific Publications

Some examples of previous publications that I have been involved in over the years (as a scientist). Some are published in peer review journals and others have appeared in academic books. The style very much reflects the services I can provide you now as a scientific writer. 

I have tried to provide downloadable versions where available (and as much as I dare to not infringe copyright!).

GC-MS methods for metabolic profiling of microbial fermentation products of dietary polyphenols in human and in vitro studies.

This project was all about developing new methods to detect polyphenols in various biological matricies.  It was published in the Journal of Chromatography B in 2008.

This is the abstract as it appeared in the journal:

Flavonoids, a subclass of polyphenols, are major constituents of many plant-based foods and beverages, including tea, wine and chocolate. Epidemiological studies have shown that a flavonoid-rich diet is associated with reduced risk of cardiovascular diseases. The majority of the flavonoids survive intact until they reach the colon where they are then extensively metabolized into smaller fragments. Here, we describe the development of GC–MS-based methods for the profiling of phenolic microbial fermentation products in urine, plasma, and fecal water. Furthermore, the methods are applicable for profiling products obtained from in vitro batch culture fermentation models. The methods incorporate enzymatic deconjugation, liquid–liquid extraction, derivatization, and subsequent analysis by GC–MS. At the level of individual compounds, the methods gave recoveries better than 80% with inter-day precision being better than 20%, depending on the matrix. Limits of detection were below 0.1 μg/ml for most phenolic acids. The newly developed methods were successfully applied to samples from human and in-vitro intervention trials, studying the metabolic impact of flavonoid intake. In conclusion, the methods presented are robust and generally applicable to diverse biological fluids. Its profiling character is useful to investigate on a large scale the gut microbiome-mediated bioavailability of flavonoids.

The article can be accessed here

Here is the full reference with all the authors listed:

Grun CH, van Dorsten FA, Jacobs DM, Le Belleguic M, van Velzen EJ, Bingham MO, Janssen HG, van Duynhoven JP (2008).  GC-MS methods for metabolic profiling of microbial fermentation products of dietary polyphenols in human and in vitro studies. Journal of Chromatography B, 871(2): 212-219

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