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Scientific Publications

Some examples of previous publications that I have been involved in over the years (as a scientist). Some are published in peer review journals and others have appeared in academic books. The style very much reflects the services I can provide you now as a scientific writer. 

I have tried to provide downloadable versions where available (and as much as I dare to not infringe copyright!).

(1)H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome.

This paper is all about the development of a comprehensive measurement tool for gut microbial metabolites.  It was then used to assess the effects of nutritional interventions on the human gut microbiome.  It was published in 2008 in NMR in Biomedicine.

Here is the abstract, as it appeared in NMR in Biomedicine:

Current research increasingly recognizes the human gut microbiome as a metabolically versatile biological digester that plays an essential role in regulating the host metabolome. Gut microbiota recover energy and biologically active molecules from food that would otherwise be washed out of the intestinal tract without benefit. In this study, a protocol for NMR-based metabolite profiling has been developed to access the activity of the microbiome. The physicochemical properties of fecal metabolites have been found to strongly affect the reproducibility and coverage of the profiles obtained. Metabolite profiles generated by water and methanol extraction of lyophilized feces are reproducible and comprise a variety of different compounds including, among others, short-chain fatty acids (e.g. acetate, propionate, butyrate, isobutyrate, isovalerate, malate), organic acids (e.g. succinate, pyruvate, fumarate, lactate), amino acids, uracil, trimethylamine, ethanol, glycerol, glucose, phenolic acids, cholate, and lipid components. The NMR profiling approach was validated on fecal samples from a double-blinded, placebo-controlled, randomized cross-over study, in which healthy human subjects consumed a placebo and either a grape juice extract or a mix of grape juice and wine extract over a period of 4 weeks, each. The considerable inter- and intra-individual variability observed originates in the first instance from variable metabolite concentrations rather than from variable metabolite compositions, suggesting that different colonic flora share general biochemical characteristics metabolizing different substrates to specific metabolic patterns. Whereas the grape juice extract did not induce changes in the metabolite profiles as compared with the placebo, the mixture of grape juice and wine extract induced a reduction in isobutyrate, which may indicate that polyphenols are able to modulate the microbial ecology of the gut.

You can access the article here

Here is the full reference with all the authors listed:

Jacobs DM, Deltimple N, van Velzen E, van Dorsten FA, Bingham M, Vaughan EE, van Duynhoven J (2008) (1)H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome.  NMR in Biomedicine. 21(6): 615-626

 

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